Part:BBa_K912023:Design

BBa_J23104(strong) promoter/RBS+ E. coli phytase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 729
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1254
  

Design Notes

None besides primer design for pcring out the e. coli phytase from the genome

Source

Constitutive promoter: registry E. coli Phytase: genomic sequence